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  • Procell-FGF-2/ bFGF/ FGF-b (K128N), Human, Recombinant

FGF-2/ bFGF/ FGF-b (K128N), Human, Recombinant

貨號:PCK005

價格:¥980¥1880¥7880¥11180

規格:
10μg
  • 10μg
  • 50μg
  • 500μg
  • 1mg
數量: - +
產品概述

產品信息

別名 Fibroblast Growth Factor 2; FGF-2; Basic fibroblast Growth Factor; bFGF; Heparin-binding Growth Factor 2; HBGF-2; FGF2; FGFB
物種 Human
表達宿主 E.coli
序列信息 Met1-Ser155
檢索號 BAG70135.1
分子量 17kDa
表觀分子量 17.2 kDa
生物活性 Testing in progress

產品特性

純度 >95% as determined by reducing SDS-PAGE.
內毒素 < 1.0 EU/μg as determined by LAL test.
保存 Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
運輸 The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
制劑 Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5.
復融 Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled waterPlease aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景介紹

Fibroblast Growth Factors (FGF) are a family of heparin-binding secreted Proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule.
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